PAL2NAL output


ERROR:  if "codon(Output format)" is selected, don't use "Remove gaps, inframe stop codons" or "Remove mismatches" or "Use only selected positions".



Synonymous (KS) and non-synonymous (KA) substitution rates calcualted by codeml in the PAML package:
KS = 2.5240
KA = 0.2252
KA/KS = 0.0892
You can see the parameters used in codeml to calculate these values.
If you use these values, please cite the following peper:
  • Goldman, N. and Yang, Z. 1994. A codon-based model of nucleotide substitution for protein-coding DNA sequences. Molecular Biology and Evolution 11:725-736.