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Global and Local Alignment of A.A Sequences



Scoring Matrices

Global Alignment

Matrix Sequences Sequence Consisting of the first four Amino Acids in the Protein GYRB_ECOL:

SNSY

Sequence Achieved by Substitution of the 2nd and 4th Amino Acid and Insertion between the 3d and 4th Amino Acid, in the Protein GYRB_ECOLI, to randomly chosen new amino acids:

TNFYV

Matrix Parameters

Match = 2
Mismatch = -1
Deletion = -2


Global Alignment Scoring Matrix


Ill.1

Alignment Responding to the Optimal Trace

SNSY-
TNFYV

Optimal Score
-1

Local Alignment

Matrix Sequences Sequence Consisting of the First Nine Amino Acids of the Protein GYRB_ECOLI:

MSNSYDSSS

Sequence Consisting of 2nd, 3d, 7th, 8th and 9th Amino Acid Residues of the Protein GYRB_ECOLI:

SNSSS

Matrix Parameters

Match = 2
Mismatch = -1
Deletion = -2


Local Alignment Scoring Matrix


Ill.2

Alignment Responding to the two Optimal Traces

MSNSYDSSS
SNS

MSNSYDSSS
SSS

Optimal Score
6

SubOptimal Score
4

Score Responding tp Alignment with Gaps
3



Influence of the Parameters on Global Alignment

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© Santesson Elly, 2004