ID   MOEB_ECOLI              Reviewed;         249 AA.
AC   P12282;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   16-DEC-2008, entry version 85.
DE   RecName: Full=Molybdopterin biosynthesis protein moeB;
GN   Name=moeB; Synonyms=chlN; OrderedLocusNames=b0826, JW0810;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=88314906; PubMed=3045084;
RA   Nohno T., Kasai Y., Saito T.;
RT   "Cloning and sequencing of the Escherichia coli chlEN operon involved
RT   in molybdopterin biosynthesis.";
RL   J. Bacteriol. 170:4097-4102(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97061202; PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
RA   Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
RA   Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
RA   Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
RA   Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
RA   Yano M., Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Involved in the biosynthesis of a demolybdo cofactor
CC       (molybdopterin), necessary for molybdoenzymes. Plays a role in the
CC       activation of the small subunit of the molybdopterin converting
CC       factor (moaD).
CC   -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
CC   -!- INTERACTION:
CC       P0A6Y8:dnaK; NbExp=1; IntAct=EBI-554435, EBI-542092;
CC       P30748:moaD; NbExp=1; IntAct=EBI-554435, EBI-554366;
CC   -!- SIMILARITY: Belongs to the hesA/moeB/thiF family.
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DR   EMBL; M21151; AAA23580.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73913.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35514.1; -; Genomic_DNA.
DR   PIR; B32352; B32352.
DR   RefSeq; AP_001457.1; -.
DR   RefSeq; NP_415347.1; -.
DR   PDB; 1JW9; X-ray; 1.70 A; B=1-249.
DR   PDB; 1JWA; X-ray; 2.90 A; B=1-249.
DR   PDB; 1JWB; X-ray; 2.10 A; B=1-249.
DR   PDBsum; 1JW9; -.
DR   PDBsum; 1JWA; -.
DR   PDBsum; 1JWB; -.
DR   DIP; DIP:10241N; -.
DR   IntAct; P12282; 15.
DR   GeneID; 945452; -.
DR   GenomeReviews; AP009048_GR; JW0810.
DR   GenomeReviews; U00096_GR; b0826.
DR   KEGG; ecj:JW0810; -.
DR   KEGG; eco:b0826; -.
DR   EchoBASE; EB0152; -.
DR   EcoGene; EG10154; moeB.
DR   HOGENOM; P12282; -.
DR   BioCyc; EcoCyc:EG10154-MON; -.
DR   BioCyc; MetaCyc:EG10154-MON; -.
DR   LinkHub; P12282; -.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; IEA:InterPro.
DR   InterPro; IPR007901; MoeZ_MoeB.
DR   InterPro; IPR012730; Mopterin_Synthase_Sase_MoeB.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   InterPro; IPR000594; ThiF_NAD_FAD_bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF05237; MoeZ_MoeB; 1.
DR   Pfam; PF00899; ThiF; 1.
DR   TIGRFAMs; TIGR02355; moeB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Molybdenum cofactor biosynthesis.
FT   CHAIN         1    249       Molybdopterin biosynthesis protein moeB.
FT                                /FTId=PRO_0000120575.
FT   HELIX         6     11
FT   HELIX        13     16
FT   TURN         19     21
FT   HELIX        22     31
FT   STRAND       33     37
FT   HELIX        41     53
FT   STRAND       56     61
FT   HELIX        68     72
FT   HELIX        79     81
FT   HELIX        86     97
FT   STRAND      101    106
FT   HELIX       112    120
FT   STRAND      122    127
FT   HELIX       132    145
FT   STRAND      149    156
FT   STRAND      158    164
FT   HELIX       173    177
FT   HELIX       194    213
FT   STRAND      220    227
FT   TURN        228    231
FT   STRAND      232    237
FT   TURN        245    247
SQ   SEQUENCE   249 AA;  26719 MW;  12C77082B3F39D7D CRC64;
     MAELSDQEML RYNRQIILRG FDFDGQEALK DSRVLIVGLG GLGCAASQYL ASAGVGNLTL
     LDFDTVSLSN LQRQTLHSDA TVGQPKVESA RDALTRINPH IAITPVNALL DDAELAALIA
     EHDLVLDCTD NVAVRNQLNA GCFAAKVPLV SGAAIRMEGQ ITVFTYQDGE PCYRCLSRLF
     GENALTCVEA GVMAPLIGVI GSLQAMEAIK MLAGYGKPAS GKIVMYDAMT CQFREMKLMR
     NPGCEVCGQ
//